Expert intracellular microelectrode assays for action potential recordings

GLP-certified expertise in cardiovascular pharmacology & electrophysiology

What are action potential recordings ?

The action potential is the consequence of the movement of ions through the different transmembrane ionic channels of the heart. To investigate the repercussion of mixed ion channel effects on cardiac electrical activity and assess cardiac safety, it is possible to record the cardiac action potential using the intracellular microelectrode technique. In vitro action potential recordings constitute convincing models of predictive drug-induced Torsades de pointes (TdP) and re-entry arrhythmias.

The microelectrode technique is not mandatory according to the ICH S7B guideline, which still focuses on hERG and QT assays and positions this model in follow-up studies. However, other factors have also been linked to the induction of severe arrhythmias. This explains why International Health agencies have been working with pharmaceutical companies for over four years now in order to revise current ICH regulations and strengthen the position of in vitro and in silico models (CiPA).

The right study model for all your action potential recordings

PhysioStim offers a wide selection of cardiac safety studies in compliance with all ICH S7A and S7B guidelines and Good Laboratory Practices. We are a preclinical Contract Research Organization of highly experienced cardiovascular pharmacology and electrophysiology experts. The experts at PhysioStim provide a wide range of possibilities to conduct both your mandatory and complementary assays for reliable and efficient cardiac safety studies.

Purkinje fiber assays

Action potential recordings in female rabbit Purkinje fibres constitute the most sensitive model to detect the effects of hERG blocking agents on cardiac repolarization.

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Rabbit atria assays

Rabbit atria can be considered a useful model to detect sodium channel blockades associated with decreases in dV/dtmax and conduction velocity.

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Papillary muscle assays

Isolated papillary muscles from guinea-pigs are standardly used in cardiovascular research to investigate the effects of drugs on KvLQT1/​MinK channels.

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Our entire range of cardiac safety assays

A wide selection for reliable preclinical safety studies

The experts at PhysioStim go beyond standard study guidelines to conduct their safety studies. We provide a wide range of possibilities to conduct both mandatory and complementary studies and help your project move forward. PhysioStim has over 17 years of expertise in developing in vitro and ex vivo models to investigate potential drug-related cardiac adverse effects. We provide cellular models as well as tissue and organ models.

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