Rabbit atria assays for preclinical safety studies

Action Potential Recordings from rabbit atria

Action potential recordings can be obtained using different tissular models. Here at PhysioStim, we use rabbit atria, rabbit or dog purkinje fibers and guinea-pig papillary muscles to answer all your study needs. The sensitivity of action potential assays differs between species and cardiac tissue types. Their selection depends on the ion channels blocked by the compound. Rabbit atria can be considered as a useful model for detection of potential sodium channel blockades associated with decreases in dV/dtmax and conduction velocity.

Why choose PhysioStim for your rabbit atria assays ?

The experts at PhysioStim respect all ICH guidelines to perform screening and GLP-compliant studies. Moreover, our stimulation protocol is designed to make sure you get the highest value out of your assay:

Technique

  • Conventional intracellular microelectrode technique

Study model

  • Rabbit atria
  • 6 preparations tested and 6 control preparations (as basic design protocol but can be adapted)
  • Options: external hypokaliemic conditions, chronic pre-​treatment, incremental stimulation rate.

Reference products

  • Quinidine 20 µmol/L
  • Flecainide 10 µmol/L

Measured parameters

  • Resting potential (mV)
  • Action Potential Amplitude (mV)
  • Action Potential Duration at 50%, 70% and 90% of repolarization: APD50, APD70, APD90 (ms)
  • Conduction time (ms)
  • Conduction speed (m/​s)
  • Maximal rate of depolarization: dV/dtmax (V/​s)

Protocol

  • 3 or 4 cumulative increasing concentrations of assay compound
  • Stimulation rates: 1 and 0.2 Hz (reverse use dependency), see above
  • Washout period or positive control at the end of the experiment
Action Potential Recordings from rabbit atria

Typical effects of flecainide effects on rabbit atria at 1 Hz

Advice from our expert scientists

In order to confirm the results of your action potential recordings and the impact on electrical activity or ECG, PhysioStim recommends completing your study with additional tests:

Need help with your preclinical safety studies ?

Please don’t hesitate to contact us