Guinea-pig cardiomyocytes for preclinical drug studies

Why use guinea-pig cardiomyocytes ?

Due to their origin, guinea-pig ventricular cardiomyocytes present the same functional and electrophysiological characteristics as native cardiomyocytes. This study model allows to test drugs on ventricular cells within the general cardiomyocyte population in order to reveal potential ventricular dysfunctions. The guinea-pig cardiomyocyte model also allows to reduce the number of animals (one animal can provide several experiments) in accordance with the 3R rule (Replacement-Reduction-Refinement).

Animal cardiomyocytes & cardiac safety

Ventricular cardiomyocytes from guinea-pigs provide a good solution to study drug effects on the models’ action potential, i.e. on ion channels. This in vitro model can be useful to evaluate the cardiac safety of newly developed drugs according to the recommendations of the European Agency for the Evaluation of Medicinal Products.

Action potential recordings

To evaluate the cardiac safety of your drug, we study its action potential, which is the consequence of ion movements through the different transmembrane ionic channels. The modification of the shape of the action potential gives information about ion channels effects.

Technique

  • Manual Patch Clamp (current clamp configuration)
  • Non-cumulative concentrations

Study model

  • Isolated ventricular cardiomyocytes by enzymatic digestion
  • 3 to 6 treated cardiomyocytes

Reference products

  • E-4031
  • Dofetilide

Measured parameters

  • Resting Potential (mV)
  • Amplitude (mV)
  • Action Potential Duration at 20% and 90% repolarisation (ADP20, ADP90) (ms)
Guinea-pig cardiomyocytes result curve

Typical effects of Dofetilide on guinea-pig ventricular cardiomyocytes

Advice from our expert scientists

In order to confirm study results and the impact on cardiac function, PhysioStim recommends completing your guinea-pig cardiomyocyte assay with additional tests:

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