PhysioStim Cardiomyocytes
PhysioStim Cardiomyocytes

Guinea-pig cardiomyocytes for preclinical drug studies

Due to their origin, guinea-pig ventricular cardiomyocytes present the same functional and electrophysiological characteristics as native cardiomyocytes. This study model allows to test drugs on ventricular cells within the general cardiomyocyte population in order to reveal potential ventricular dysfunctions. The guinea-pig cardiomyocyte model also allows to reduce the number of animals (one animal can provide several experiments) in accordance with the 3R rule (Replacement-Reduction-Refinement).

Animal cardiomyocytes & cardiac safety

Ventricular cardiomyocytes from guinea-pigs provide a good solution to study drug effects on action potential. This in vitro model is well established in cardiac safety studies as it is included in the ICH S7B guideline for in vitro eletrophysiology follow-up studies. It can be useful to evaluate the cardiac safety of newly developed drugs according to the recommendations of the European Agency for the Evaluation of Medicinal Products.

What is shown?

To evaluate the cardiac safety of your drug, we study its effects on the shape of the action potential. A modification of the shape of the action potential gives information about ion channels effects.

Technique

  • Manual Patch Clamp (current clamp configuration)
  • Non-cumulative concentrations

Study model

  • Isolated ventricular cardiomyocytes by enzymatic digestion
  • 3 to 6 treated cardiomyocytes

Measured parameters

  • Resting Potential (mV)
  • Amplitude (mV)
  • Action Potential Duration at 20% and 90% repolarisation (ADP20, ADP90) (ms)

Stimulation protocol

Guinea-pig cardiomyocytes

Typical effects of Dofetilide on guinea-pig ventricular cardiomyocytes.

Reference products

  • E-4031
  • Dofetilide

Additional tests according to PhysioStim scientist’s expertise

Humans cardiomyocites

Human Cardiomyocytes

PhysioStim Isolated Perfused Heart

Isolated perfused heart

hERG assays

PhysioStim Sodium Channel assays

Sodium channel assays

Calcium channel

Calcium channel assays

PhysioStim Potassium Channel assays

Other Potassium channel assays

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