PhysioStim Rabbit attria assays
PhysioStim Rabbit attria assays mobile

Rabbit atria assays for preclinical safety studies

Action potential recordings can be obtained using different tissular models. Here at PhysioStim, we use rabbit atria, rabbit or dog purkinje fibers and guinea-pig papillary muscles to answer all your study needs. The sensitivity of action potential assays differs between species and cardiac tissue types. Their selection depends on the ion channels blocked by the compound. Rabbit atria can be considered as a useful model for detection of potential sodium channel blockades associated with decreases in dV/dtmax and conduction velocity.

Technique

  • Conventional intracellular microelectrode technique

Study model

  • Rabbit atria
  • 6 preparations tested and 6 control preparations (as basic design protocol but can be adapted)
  • Options: external hypokaliemic conditions, chronic pre-treatment, incremental stimulation rate.

Measured parameters

  • Resting potential (mV)
  • Action Potential Amplitude (mV)
  • Action Potential Duration at 50%, 70% and 90% of repolarization: APD50, APD70, APD90 (ms)
  • Conduction time (ms)
  • Conduction speed (m/s)
  • Maximal rate of depolarization: dV/dtmax (V/s)

Stimulation protocol

  • 3 or 4 cumulative increasing concentrations of assay compound
  • Stimulation rates: 1 and 0.2 Hz (reverse use dependency)
  • Washout period or positive control at the end of the experiment

Results

Rabbit Atria Protocol

Typical effects of flecainide on rabbit atria at 1 Hz

Ref­er­ence compounds

Reference compoundsConcentration

Quinidine 20 µmol/L

Flecainide 10 µmol/L

Additional tests according to PhysioStim scientist’s expertise

Humans cardiomyocites

Human Cardiomyocytes

PhysioStim Isolated Perfused Heart

Isolated perfused heart

hERG assays

PhysioStim Sodium Channel assays

Sodium channel assays

Calcium channel

Calcium channel assays

PhysioStim Potassium Channel assays

Other Potassium channel assays

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